Journal article

Human papillomavirus genotype detection from Archival Papanicolaou-stained cervical tests

SN Tabrizi, N Taylor, MJ McCullough, G Phillips, J Wark, D Gertig, RW Petersen, M Saville, SM Garland

Cancer Cytopathology | WILEY | Published : 2010

Abstract

BACKGROUND: Archival Papanicolaou (Pap)-stained cervical cytology tests may be the only source of a clinical sample for the evaluation of previous human papillomavirus (HPV) infection. Pap tests are ideal because the majority of women in countries with comprehensive screening programs would have had several collected and stored. METHODS: In the current study, HPV detection and genotyping were compared in samples collected from a conventionally fixed Pap test with those collected using an endocervical brush and collected in PreservCyt (liquid-based) in 87 women undergoing management for a high-grade Pap test abnormality. Cytology slides were scanned to create high-resolution digital images be..

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